Fig. 6
C/EBPβ involved in the regulation of hypoxia-induced cell proliferation. a quantitative RT-PCR demonstrated the decrease of the C/EBPβ mRNA level in RA-FLS cultured in 3% O2 hypoxia for 24 h. b Western blot demonstrated the downregulation of C/EBPβ at the protein level in RA-FLS cultured in 3% O2 hypoxia for 48 h. c Cell proliferation capacity was determined by CCK8 assay in RA-FLS overexpressed with C/EBPβ cultured under condition of normoxia or hypoxia. (A: Normoxia+pEnter-ctrl versus Hypoxia+pEnter-ctrl, p < 0.05; B: Hypoxia+pEnter-ctrl versus Hypoxia+pEnter-C/EBPβ, p < 0.05). All experiments were repeated three times. Data were presented as mean ± SEM (n = 3, *p < 0.05 by t test)