Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Antifibrotic effects of 2-carba cyclic phosphatidic acid (2ccPA) in systemic sclerosis: contribution to the novel treatment

Fig. 5

2ccPA regulated the fibrogenic environment in part by increasing intracellular cAMP levels in SSc skin fibroblasts. a SSc skin fibroblasts (n = 5) were incubated with either 0–10 μM 2ccPA for 30 min in the presence of 3-isobuthyl-1-methylxanthine. One micromolar of forskolin was administrated in SSc skin fibroblasts (n = 3) as a positive control. The intracellular cAMP levels were assessed using an enzyme immunoassay kit. b Profibrotic markers were downregulated by increasing intracellular cAMP levels in SSc skin fibroblasts. SSc skin fibroblasts (n = 5) were incubated in the presence of 0–10 μM forskolin for 48 h. COL1A1, COL1A2, CTGF, and ACTA2 mRNA was assessed by qPCR. Data are the representative of three independent experiments. The bars represent as median with IQR. *p < 0.05

Back to article page