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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Depletion of activated macrophages with a folate receptor-beta-specific antibody improves symptoms in mouse models of rheumatoid arthritis

Fig. 1

Staining of FR-β-positive macrophages in tissues from patients with inflammatory/autoimmune diseases. a Tissue sections from patients with psoriasis, scleroderma, and sarcoidosis were stained with Hoescht dye (DNA, blue channel), anti-CD68 antibody followed by Cy3 labeled secondary antibody (macrophages, false colored green), and anti-FR-β antibody followed by Cy5 labeled secondary antibody (FR-β, false colored red). Tissue sections were imaged via fluorescent microscopy at 400×. b Representative images of IHC staining for FR-β in human tissue sections from patients with Crohn’s disease (n = 12), ulcerative colitis (n = 9), normal lung (n = 31), idiopathic pulmonary fibrosis (IPF; n = 53), nonspecific interstitial pneumonia (NSIP; n = 32), chronic obstructive pulmonary disease (COPD; n = 40), rheumatoid arthritis (RA; n = 30), systemic lupus erythematosus (Lupus; n = 6), psoriasis (n = 8), and scleroderma (n = 4). c Images were analyzed and the mean staining intensity (gray value), percentage of the total area staining positive (% of pixels above threshold), and staining score (staining intensity multiplied by % of area staining positive) were plotted. Error bars represent SEM for all panels

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