Fig. 1

Chemokines bind to soluble syndecan-3 and heparan sulfate (HS). Isothermal fluorescence titration (A+B). a HS binding isotherms of CCL2 (black squares), CCL7 (red circles) and CXCL8 (blue triangles). b Syndecan-3 binding isotherms of the same chemokines. For K_d values, see Table 1. The y-axis shows the relative change in fluorescence intensity following ligand addition: ΔF = F (fluorescence emission at a certain ligand concentration) − F0 (fluorescence emission in the absence of ligand). c Syndecan-3 and HS were coated onto GAG microplates overnight [25 μg/m]. A dilution series of CCL7 was added to the plates to identify the level of binding. Plates were read at 405 nm. Data points represent means ± standard error (n = 3 separate experiments)