Fig. 4

Effects of TNFα on syndecan-3 shedding. a Endothelial cells were treated with or without TNFα [100 ng/ml] supplemented media for up to 24 h. The media was then subjected to an ELISA to detect syndecan-3. The graph depicts TNFα-stimulated shedding values minus those of controls in the absence of TNFα at each time point. The bars represent mean values with standard errors (n = 3 independent experiments). The stars represent a significant ANOVA followed by Tukey post-test (P < 0.05). b Again, endothelial cells were cultured with and without TNFα-supplemented media. This time, the level of intracellular and extracellular syndecan-3 was assessed via flow cytometry in comparison with cells treated with control media. Data points represent percentage change of cells positive for syndecan-3 intracellularly or on the extracellular surface (fluorescence intensity; means with standard errors; n = 3 independent experiments). The three stars represent a significant two-way ANOVA (without repeated measures) with Tukey post-test (***P < 0.001) depicting a highly significant difference between intracellular and extracellular expression at 24 h. The plus symbol represents significant differences (via one-way ANOVA with Tukey post-test) within the extra- and intracellular expression profiles at 24 h (⁺⁺P < 0.01)