Fig. 2

Platelet activation in the presence of anti-D4GDI antibodies from the sera of PAPS patients. Preincubation of platelets from healthy donors with 20 μg/ml of anti-D4GDI antibodies, purified from the sera of PAPS patients, significantly increases the a total % (n = 5) and the b kinetic rate (representative image, n = 5) of adenosine diphosphate (ADP)-induced integrin activation (gray line and bar), compared to controls with buffer only (black line and bar). In the absence of agonist stimulation (none), there is a similar trend but is not statistically significant