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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Chondrogenic induction of human osteoarthritic cartilage-derived mesenchymal stem cells activates mineralization and hypertrophic and osteogenic gene expression through a mechanomiR

Fig. 6

MiR-365 expression in human cartilage cells; and protein expression of MSC marker CD166, hypertrophic cartilage markers Col10a1 and Mmp13 in articular cartilage of miR-365 transgenic mice (Tg). a A bar graph showing quantification of miR-365 expression in normal cartilage progenitor cell line 3 (nCPC3), primary OA-MSC, and primary OA chondrocytes (OAC) respectively. Representative immunohistochemistry staining of CD166 (b, c), Col10a1 (e, f), and Mmp13 (h, i) in 6-month-old miR-365 Tg mice (MiR-365 fl +/−; Col2a1-Cre+/−) and age-matched control Cre-only mice (MiR-365 fl −/−; Col2a1-Cre+/−) knee articular cartilage. d Quantification of CD166 positive cell numbers in b and c. g Quantification of Col10a1 positive tissue areas in e and f. j Quantification of Mmp13 positive tissue areas in h and i. k, l Representative Safranin O staining of 6-month-old miR-365 Tg mice (MiR-365 fl +/−; Col2a1-Cre+/−) and age-matched control Cre-only mice (MiR-365 fl −/−; Col2a1-Cre+/−) knee articular cartilage. m OARSI scoring of k and l. Arrows point to the immuno-positive signals. The images are representative of analyses of at least three mice per group. Data are presented as mean ± SD from at least 3 sample images for each group; *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001, relative to indicated control groups respectively

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