Fig. 5

Tofacitinib facilitates the expansion of MDSCs in vitro. Lung-infiltrating cells (1 × 10⁶) (a, b) and BM cells (1 × 10⁶) (c, d) from naïve SKG mice were cultured with 100 ng/ml GM-CSF and 2.5 ng/ml rIL-4 for 5 days with or without tofacitinib. a Representative flow cytometry plots and the comparison of MDSCs and CD11b⁺Gr1^dim cells differentiated from lung-infiltrating cells cultured with or without tofacitinib (tofacitinib, n = 5; control, n = 5). b Representative flow cytometry plots and the comparison of CD11b⁺CD11c⁺ cells and CD11b^dimCD11c⁺ cells differentiated from lung-infiltrating cells cultured with or without tofacitinib. c Representative flow cytometry plots and the comparison of MDSCs differentiated from BM cells cultured with or without tofacitinib (tofacitinib, n = 5; control, n = 5). d Representative flow cytometry plots and the comparison of BMDCs differentiated from BM cells cultured with or without tofacitinib. Data are shown as the mean ± SEM. *P < 0.05, Student’s t tests