Table 1 Sequences of primers used for quantitative real-time PCR
Gene | Forward (5–3′) | Reverse (5–3′) |
|---|---|---|
HOTAIR | CATTCTGCCCTGATTTCCG | ATCCGTTCCATTCCACTGCG |
MMP3 | TTCCTTGGATTGGAGGTGAC | AGCCTGGAGAATGTGAGTGG |
MMP9 | CAGTCCACCCTTGTGCTCTTCCCTG | ATCTCTGCCACCCGAGTGTAACCA |
MMP10 | CAGGTTATCCAAGAGGCATCCATAC | TTAGGCTCAACTCCTGGAAAGTCAT |
Type II collagen | TCCAGATGACCTTCCTACGC | GGTATGTTTCGTGCAGCCAT |
Aggrecan | TGAGCGGCAGCACTTTGAC | TGAGTACAGGAGGCTTGAGG |
Wnt1 | GAATCGCCGCTGGAACTGTC | GCGGAGGTGATAGCGAAGATAAACG |
β-catenin | CAAGTGGGTGGTATAGAGG | AGTCCATAGTGAAGGCGAAC |
GAPDH | TCAAGAAGGTGGTGAAGCAGG | TCAAAGGTGGAGGAGTGGGT |