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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: JAG2/Notch2 inhibits intervertebral disc degeneration by modulating cell proliferation, apoptosis, and extracellular matrix

Fig. 2

The regulating role of Notch signaling in NP cell proliferation. a JAG2 induced the proliferation of NP cells, and depletion of Notch2/Hey2 reduced NP cell proliferation. b The efficiency of the Notch2/Hey2/Hes1 siRNAs. c, d Cyclin D1 and CDK6 mRNA and protein expression levels were increased by recombinant JAG2 treatment and decreased by silencing of Notch2, Hes1, or Hey2. fh Cyclin D1 and CDK6 protein expression levels were increased by recombinant JAG2 treatment and decreased by silencing of Notch2, Hes1, or Hey2. ei p21 mRNA and protein expression levels were decreased by recombinant JAG2 treatment and increased by silencing of Notch2, Hes1, or Hey2. j Luciferase reporter assays confirmed that the activity of the cyclin D1 promoter was significantly upregulated by recombinant JAG2 treatment and downregulated by Notch2/Hey2/Hes1 siRNA. P values were computed vs. non-stimulated controls* or JAG2-stimulated controls#; *,#P < 0.05

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