Fig. 6

Notch inhibited TNF-α-promoted apoptosis via the RIP1-FADD-caspase-8 complex. a, b The apoptotic rate of NP cells treated with Notch2 siRNA plus TNF-α was decreased by the silencing of RIP. c, d z-IETD-fmk inhibited Notch2 siRNA-promoted cell death and apoptosis. e-h Real-time PCR and Western blotting showed that cleaved-caspase-3 and its substrate PARP were inhibited in Notch2 siRNA plus TNF-α-treated NP cells in the presence of z-IETD-fmk. i The efficiency of the RIP siRNA. P values were computed vs. non-stimulated controls*, TNF-α-stimulated controls^#, or TNF-α and Notch2 siRNA-stimulated controls^&; *^,#,&P < 0.05