Table 1 Primer sequences for related genes
Gene | Primer Sequences (5′–3′) |
|---|---|
Rat NOTCH1 | Forward: ACGCCTACCTCTGCTTCTGC Reverse: CAGGCACACTCGTAGCCATC |
Rat NOTCH2 | Forward: ATGTGTCAACGGCTGGAGTG Reverse: GCAAGAGAAGGAGGCCACAC |
Rat NOTCH3 | Forward: GCGAGGCAGACATCAATGAG Reverse: ACAGCGAGGACCTGAGAAGC |
Rat NOTCH4 | Forward: GAAGGCCACAGACACAGCAG Reverse: CACATGACCACTCCGTCCTC |
Rat JAG1 | Forward: CTGTGGCTTGGATCTGTTGC Reverse: CGTTGTTGGTGGTGTTGTCC |
Rat JAG2 | Forward: ATCTGTGAGGACCTGGTGGATGG Reverse: GTAGCAGCGAGCACCGTTGAG |
Rat delta-like1 | Forward: AGCCTCCGCCTGATCCTTGC Reverse: TCCGCCTTCTTGTTGGTGTTCTTG |
Rat delta-like 3 | Forward: CTGCCTTGTCGTTGCCTGATGG Reverse: GCCACACGCGCTAATAGGTTCC |
Rat delta-like 4 | Forward: AAGCATTACCAGGCAACCTTCTCC Reverse: TTACGACCACTGCCGCTATTCTTG |
Rat Hes1 | Forward: CCAAGCTGGAGAAGGCAGACATTC Reverse: GGTCACCTCGTTCATGCACTCG |
Rat Hes5 | Forward: GACCGCATCAACAGCAGCATTG Reverse: TCTCCAGGATGTCGGCCTTCTC |
Rat Hey1 | Forward: CTGTCGCCGCCATGCTTCTC Reverse: GCTGCCTGTGAGGTGTCAAGAC |
Rat Hey2 | Forward: CTAGAGAGGACCTGGAGAGTTTAAG Reverse: CTGTGCCACCAGCCTTAAAACC |
Rat CBF1 | Forward: GGTGTGAGGAGGAGGAACAATGAC Reverse: GCACGAGCAGCCATCTCAGC |
Rat cyclin D1 | Forward: CCTGACACCAATCTCCTCAACGAC Reverse: TTCCGCATGGATGGCACAATCTC |
Rat CDK6 | Forward: CTCTGAAGCGCGTGCGAGTG Reverse: AAGGTCTCCAGGTGCCTCAGC |
Rat p21 | Forward: GTTCCTTGTGGAGCCGGAGC Reverse: GGTACAAGACAGTAGCAGATC |
Rat PARP | Forward: CTAAGTGTTCGTCTTTAG Reverse: AGAAATTGTTAGCGTTCC |
Rat PI3K | Forward: CAATGATGCTTGGCTCTGGAATGC Reverse: TTGTCCAGCCACCATGATGTGC |
Rat Fas | Forward: GGCGGGTTCGTGAAACTGAT Reverse: AGGTTGGCATGGTTGACAGC |
Rat RIP | Forward: AGCCTGGTTCACTGCACAGTTC Reverse: ATGGTAGTTGGCTTCGTCTTGGAG |
Rat β-tubulin | Forward: CAGCGATGAGCACGGCATAGAC Reverse: CCAGGTTCCAAGTCCACCAGAATG |
Rat β-actin | Forward: CGTCCGTGACATCAAGGAGAAGC Reverse: ACCGAGGAAGGAAGGCTGGAAG |