Fig. 2

Altered number and activation status of microglia and CD11b⁺ myeloid cells in the CNS of lupus model mice. a Representative plots of CD45⁺ cells in brains by flow cytometry. Cells were characterized as CD11b⁻ cells, CD11b⁺ myeloid cells, and microglia. b Number of total brain microglia and CD11b⁺ myeloid cells. c Representative histograms of MHC class I expression in microglia and CD11b⁺ myeloid cells. Red histograms represent lupus model mice, and blue histograms represent their controls. d Comparison of MHC class I expression in lupus model mice and their controls. e Representative plots of PDCA1 expression in microglia and CD11b⁺ myeloid cells. Percentages of positive cells are shown. f Comparison of PDCA1-positive cells in lupus model mice and their controls. In b, d, and f, symbols represent individual mice (n = 8 for B and D, n = 6 for F for FcγRIIB^−/−Yaa mice and controls, n = 6 for NZB/NZW mice and controls, n = 4 for MRL/lpr mice and controls) and horizontal lines indicate the mean and SEM. *P < 0.05, **P < 0.01, and ***P < 0.01 by Student’s t test