Fig. 3
Effect of PlGF-2123-144 conjugation on the recognition of α-TNF against the target antigen. a Blocking affinities of unmodified α-TNF and PlGF-2123-144-α-TNF to TNFα were analyzed by ELISA (n = 3, mean + SE). b Osteoclast precursor RAW264.7 cells were incubated with TNFα and either unmodified α-TNF or PlGF-2123-144-α-TNF for 7 days, and then differentiated osteoclasts were stained by TRAP kit. Percent inhibition of osteoclast differentiation calculated from the ratio of the number of osteoclasts in each treatment to that in the untreated control (n = 6, mean ± SE). c Representative images of osteoclast differentiation study taken at 5-fold magnification