Fig. 6

In vitro immunosuppressive potency of CD4⁺CD25⁺ T cells assessed by allogeneic mixed lymphocyte reaction in active SLE patients and healthy controls. Allogeneic peripheral blood mononuclear cells (allo-PBMCs) were cocultured with flow cytometer-sorted CD4⁺CD25⁺ cells, which were regarded as T regulatory cells (Treg cells), and/or CD4⁺CD25⁻ cells, which were regarded as effector T cells (Teff cells). Cell proliferation was quantitatively assessed using CSFE labeling. PHA was used as a control for cell proliferation. a Representative histograms for cell proliferation in a healthy donor and a patient with SLE. b Immunosuppressive activity of CD4⁺CD25⁺ T cells in active SLE patients (n = 5) and healthy controls (n = 5). NS = not significant