Fig. 4

Serum CORO1A concentration levels segregate LN patients, from SLE patients without nephritis and healthy controls. Absolute quantification of Coronin-1A concentration levels in sera from LN patients (n = 16), SLE patients (n = 18) and healthy controls (n = 24), using targeted MRM-MS. a Bland-Altman plot comparing CORO1A mean concentration levels between LN cases, SLE cases and healthy controls. The median serum Coro1A concentrations were determined as 21.47 (interquartile range (IQR) 15.49–31.54), 17.13 (IQR 16.5–19.47) and 47.02 (IQR 43.085–54.84) ng/ml in the serum of healthy controls (HC), SLE and LN, respectively. Statistical analysis was performed using non-parametric tests. The three-group analysis was performed using the Kruskal-Wallis rank test (p value = 0.0001), while the two-group analyses using the Wilcoxon rank-sum test (HC vs SLE, p value = 0.19; HC vs LN, p value < 0.00001; SLE vs LN, p value < 0.00001). b Bland-Altman plot indicating that K-means based consensus clustering classifies LN patients and healthy controls into two distinct groups, shown as Group B and Group A according to their serum CORO1A concentration levels. c Receiver-operating characteristic (ROC) curve analysis for serum CORO1A, showing high specificity (91.67%) and sensitivity (100%) of the CORO1A to correctly detect incidences of LN and controls. Area under the curve = 0.9792, 95% confidence interval = 0.94–1.00, optimal cut-off 37.24 ng/ml. d Bland-Altman plot indicating that K-means based consensus clustering classifies LN and SLE cases into two distinct groups, shown as Group B and Group A according to their serum CORO1A concentration levels. e ROC curve analysis for serum CORO1A, showing high specificity (100%) and sensitivity (100%) of the CORO1A to correctly detect incidences of LN and SLE cases. Area under the curve = 1.00, 95% confidence interval = 1.00–1.00, optimal cut-off 37.24 ng/ml. Vertical lines present the median and interquartile range