Fig. 4

DNA vaccination of NZB/W mice with pHSP90 associates with the induction of tolerogenic immune responses a Increased frequency of CD4⁺CD25⁺Foxp3⁺ Tregs in popliteal lymph nodes from mice that had been DNA-vaccinated with pHSP90 as compared to mice receiving pHSP60, empty plasmid (pcDNA3), or PBS. Monitoring was done after DNA vaccination on a weekly basis, ex vivo, by flow cytometry. b Concentration of serum cytokine levels at 24 weeks in DNA-vaccinated mice. *P < 0.05 vs. control pcDNA3