Fig. 3

CKD-506 augments Treg-mediated suppression of T cell proliferation. Induced Treg cells (iTreg) from RA patients (n = 3) were co-cultured for 72 h at different ratios with CFSE-labeled T cells from healthy controls in the presence of an HDAC6 inhibitor and CD3/28 Dynabeads, and the proliferation of T cells was examined by FACS. a Representative FACS data from three independent experiments are shown. b T cell proliferation under different ratio of iTreg to T cells. c Changes in expression of CTLA4 by iTreg and T cells after treatment with CKD-506. *p < 0.05, **p < 0.01, ***p < 0.001 vs. no treatment