Fig. 3

Differential gene analyses reveal discrete transcriptomic signature of chondroclasts and osteoclasts. The raw hybridization data were normalized using quantile normalization to the 75th percentile, and genes with differential expression levels greater than 2-fold (FDR P value < 0.05) were subjected to scatter plot (a), volcano plot (b), and heatmap analysis (c). There is a clear dichotomy between the genomic signatures of the chondroclasts and osteoclasts. d Validation of microarray results by quantitative PCR. Select genes were chosen for further analysis and validation of the microarray hybridization results. RNAs were reisolated from chondroclasts and osteoclasts captured by LCM from femurs harvested 12 days post-fracture and analyzed by qPCR for expression of genes. The results compare the levels of expression of the qPCR and the microarray hybridization