Fig. 3

OSM and IL-6+sIL-6R stimulate transition to mesenchymal phenotype in HDMECs. a mRNA levels of EndMT genes were analyzed by quantitative PCR, n = 3. Student’s t test *p < 0.05, **p < 001, ***p < 0.0001. b Immunofluorescence staining of VE-cadherin (green) and phalloidin (red) in the HDMECs culture treated with OSM and IL-6+sIL-6R. Representative images are shown from 3 cell lines. c Representative western blot of VE-cadherin, CD31, αSMA, and TGFβ123 in HDMECs treated with OSM and IL-6+IL-6R for 24 and 48 h. N = 2