Fig. 5

Treatment with IL13Rα1 halts and reverses the development of CIA. a The synovial tissues from knee joints in Lv-IL13Rα1 or Lv-Ctrl transfected CIA mice (n = 10 per group) at the resolution phase of arthritis were homogenized in cell lysis buffer for Western blot detection of IL13Rα1, DR5, Trail, Bax, Bcl, and GFP (left). Band intensities are quantified and the Bax/Bcl ratio is summarized into bar charts (right). b, c Comparison of the clinical score (b) and paw swelling (c) between arthritic mice treated with Lv-Myc-IL13Rα1 (n = 10) or Lv-Ctrl (n = 10). d Representative images of hematoxylin-eosin (H&E, upper) and Safranin O/Fast green staining (lower) of the knee joint. The synovial thickening (red arrow) and bone erosion (red asterisk) are shown (n = 10). e Inflammation, hyperplasia, cartilage degradation, and bone destruction as evaluated through the scoring system (n = 10 per group). f FLSs were isolated from knee joints of CIA models (n = 6 per group) and subjected to flow cytometry for analyzing apoptosis. *p < 0.001 vs the mean ± SD of indicated parameters in Lv-Ctrl