Table 1 Primers to measure expression in synovium and/or DRG using Q-PCR
From: The alarmins S100A8 and S100A9 mediate acute pain in experimental synovitis
Gene | Forward primer | Reverse primer |
|---|---|---|
α2δ1(A2d1) | 5′-gtcacactggattttctcgatgc-3′ | 5′-gggtttctgaatatctggcctga-3′ |
Atf3 | 5′-gaggattttgctaacctgacacc-3′ | 5′-ttgacggtaactgactccagc-3′ |
Cfos | 5′-aaacccatcaccatcttcca-3′ | 5′-gtggttcacacccatcacaa-3′ |
Cgrp | 5′-ttgtcagcatcttgctcctgtac-3′ | 5′-gcctgggctgctttcca-3′ |
Galanin | 5′-ggcagcgttatcctgctagg-3′ | 5′-ctgttcagggtccaacctct-3′ |
Gap43 | 5′-tggtgtcaagccggaagataa-3′ | 5′-gctggtgcatcacccttct-3′ |
Gapdh | 5′-ggcaaattcaacggcaca-3′ | 5′-gttagtggggtctcgctcctg-3′ |
Il1b | 5′-ggacagaatatcaaccaacaagtgata-3′ | 5′-gtgtgccgtctttcattacacag-3′ |
Il6 | 5′-caagtcggaggcttaattacacatg-3′ | 5′-attgccattgcacaactcttttct-3′ |
Kc | 5′-tggctgggattcacctcaa-3′ | 5′-gagtgtggctatgacttcggttt-3′ |
Mcp1 | 5′-ttggctcagccagatgca-3′ | 5′-cctactcattgggatcatcttgct-3′ |
Nav1.7 | 5′-cgacagcggcacaactaatc-3′ | 5′-agaatgcttgctctgctcatg-3′ |
Ngf | 5′-tcgggccagtatagaaagct-3′ | 5′-ggggagcgcatcgagtttt-3′ |
Npy | 5′-atgctaggtaacaagcgaatgg-3′ | 5′-tgtcgcagagcggagtagtat-3′ |
P2rx3 | 5′-aaagctggaccattgggatca-3′ | 5′-cgtgtcccgcacttggtag-3′ |
S100a8 | 5′-tgtcctcagtttgtgcagaatataaat-3′ | 5′-tttatcaccatcgcaaggaactc-3′ |
S100a9 | 5′-ggcaaaggctgtgggaagt-3′ | 5′-ccattgagtaagccattcccttta-3′ |
SubstanceP (Tac1) | 5′-attcctttgttggactaatgggc-3′ | 5′-acgtcttctttcgtagttctgc-3′ |
Tnfa | 5′-cagaccctcacactcagatcatct-3′ | 5′-cctccacttggtggtttgcta-3′ |
Trka | 5′-gcctaaccatcgtgaagagtg-3′ | 5′-ccaacgcattggaggacagat-3′ |