Fig. 1

Gating strategy for flow cytometry sorting of human CD14⁺CD16⁺ and CD14⁺CD16⁻ monocytes. Peripheral blood mononuclear cells from RA and HC were stained with FITC-conjugated anti-CD14 antibody and APC-conjugated anti-CD16 antibody. CD14⁺CD16⁺ and CD14⁺CD16⁻ monocytes were analyzed and sorted by flow cytometric; the purity of sorted CD14⁺CD16⁺ and CD14⁺CD16⁻ monocytes used for experiments was ~ 90%