Fig. 4

Intracellular signaling pathways involving in the regulation of IL-25 in IL-22-induced RANKL expression in PBMC. a Immunoblotting of p-stat3, stat3, p-P38, P38, p-IκB-α, IκB-α, and beta-actin in the PBMC pre-treated with IL-25 (10, 50, 100 ng/ml) and then cultured under IL-22 for 1 h. b Data were normalized to beta-actin and reported in relative expression units. Bars show the mean ± SEM of 3 independent experiments. ***P < 0.001