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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: MicroRNA-15b shuttled by bone marrow mesenchymal stem cell-derived extracellular vesicles binds to WWP1 and promotes osteogenic differentiation

Fig. 2

BMSC-secreted EVs mediated delivery of miR-15b reduces WWP1 expression in BMSCs. a The structure of EVs observed under a TEM (scale bar = 100 nm). b The size of EVs detected by NTA. c The expression of CD63, CD81, Calnexin, and TSG101 in EVs and lysate detected by Western blot analysis normalized to GAPDH. d miR-15b expression in BMSCs after co-culture with BMSC-derived EVs determined by RT-qPCR normalized to U6. *p < 0.05 vs. hBMSCs. e The uptake of EVs by BMSCs observed under a fluorescence microscope (× 400). f miR-15b expression in BMSCs after co-culture with inhibitor-NC, EV-inhibitor-NC, or EV-miR-15b inhibitor determined by RT-qPCR normalized to U6. g WWP1 expression in BMSCs after co-culture with inhibitor-NC, EV-inhibitor-NC, or EV-miR-15b inhibitor determined by Western blot analysis normalized to GAPDH. *p < 0.05 vs. hBMSCs treated with inhibitor NC. #p < 0.05 vs. hBMSCs treated with EV-inhibitor-NC. The measurement data were shown as mean ± standard deviation. Data between the two groups were compared by independent sample t test. Comparisons among multiple groups were performed using one-way ANOVA, followed by Tukey’s post hoc test. The cell experiment was repeated three times

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