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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

Fig. 3

Detailed t-distributed stochastic neighbor embedding (t-SNE) plots comparing transcriptomic expression between the three patients (P1, P2, and P3) and the preservation method (CryoStor® vs. RPMI) demonstrate even dispersion among cell clusters. a Three patients overlap well with cellular transcriptomic expression across the 18 cell clusters. b CryoStor® (frozen) and RPMI (fresh) preservation methods show even dispersion across cell clusters. c Individual patient with paired frozen and fresh specimens demonstrate even dispersion. These t-SNE plots represent 14,901 skin cells, derived from 3 patients with localized scleroderma (3 cryopreserved and 3 fresh samples with 6242 and 8659 cells, respectively)

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