Single-cell transcriptome conservation in a comparative analysis of fresh and cryopreserved human skin tissue: pilot in localized scleroderma

Table 1 Single-cell sequencing performance metrics are equivalent between skin specimens preserved in frozen media (CryoStor® CS10) compared to fresh media (RPMI)

Patient	Preservation method	Mean reads per cell	Median genes per cell	Sequencing saturation (%)
P1	CryoStor	93,607	1023	89
P1	RPMI	52,979	1353	76
P2	CryoStor	114,765	1135	89
P2	RPMI	53,131	1410	75
P3	CryoStor	104,331	896	87
P3	RMPI	163,811	710	94

ISSN: 1478-6362