Heparan sulfate functions are altered in the osteoarthritic cartilage

Table 2 IC₅₀ of total GAG, HS, and the mix of CS and KS for FGF2 and VEGF. IC₅₀ were determined in μg/mL and defined as the concentration of tested cartilage GAG able to compete with heparin for 50% of the binding to HBP (FGF2 and VEGF), and used to calculate the % of binding affinity shown in Fig. 3

IC50 (μg/ml)	CT	Femur	Tibial Plateau	Patella	OA
Total GAG
FGF-2	11 ± 4	88 ± 23	75 ± 23	91 ± 1	84 ± 20
VEGF	NoC	113 ± 76	187 ± 125	106 ± 36	136 ± 78
HS
FGF-2	34 ± 14	74 ± 31	88 ± 8	110 ± 30	82 ± 25
VEGF	13 ± 2	39 ± 19	45 ± 3	21 ± 2	38 ± 12
CS + KS
FGF-2	NoC	NoC	NoC	NoC	NoC
VEGF	NoC	NoC	NoC	NoC	NoC

Values correspond to the mean ± SEM for the CT group (n = 7) compared to the different parts of OA cartilages: femur (F), tibial plateau (TP), and patella (P) and to all OA cartilage parts (OA, n = 11). Each GAG sample was analyzed in duplicate in three independent experiments. NoC no competition, HS heparan sulfate, KS keratan sulfate, CS chondroitin sulfate

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