Fig. 3

Signalling pathways involved in IL-6-regulated protein synthesis of Cyr61 in rheumatoid arthritis-fibroblast-like synoviocytes (RA-FLSs). a, c, e Cells were pretreated with inhibitors for 2 h before IL-6 (20 ng/mL) stimulation for 2 h. LY294002 (10 μM): PI3K/AKT inhibitor, AG490 (50 μM): JAK2/STAT3 inhibitor, SB203580 (10 μM): p38 MAPK inhibitor, PD98059 (1 μM): ERK inhibitor. d, f RA-FLSs transfected with either small interfering RNA (c-Jun or EGR3) or siNC (control) (20 pmol/L) stimulated by IL-6 (20 ng/mL) for 2 h. Data are representative of at least three independent experiments. a–f Protein levels were determined by western blotting. FLSs were incubated overnight in 1% FBS-containing medium before treatment with IL-6/sIL-6R