Fig. 5
From: HDAC2 interacts with microRNA-503-5p to regulate SGK1 in osteoarthritis
miR-503-5p downregulates SGK1 expression in OA. a Prediction of potential target genes of miR-503-5p by the miRanda database (http://www.microrna.org/microrna/home.do), the mirDIP database (http://ophid.utoronto.ca/mirDIP/index.jsp#r), and the miRDB database (http://www.mirdb.org/). b Binding site between miR-503-5p and SGK1. c Expression of SGK1 in OA samples and normal samples in microarray dataset GSE46750. d Binding relationship between miR-503-5p and SGK1 confirmed by dual luciferase reporter gene assay. e RT-qPCR determination of SGK1 expression in chondrocytes after overexpressing/silencing miR-503-5p. f Western blot analysis of SGK1 expression in chondrocytes after overexpressing/silencing miR-503-5p. *p < 0.05. #p < 0.05. Measurement data were expressed as mean ± standard deviation. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. The experiment was repeated three times