Fig. 3

HOTAIR drives GLI2 transcription through EZH2 in HOTAIR-expressing and SSc fibroblasts. RNA and protein were extracted from scramble and HOTAIR-expressing fibroblasts. In addition, the scramble and HOTAIR-expressing fibroblasts were treated with the EZH2 inhibitor GSK126. a GLI2 transcript levels were analysed. Graph represents the mean and standard error for three independent experiments. b GLI2, α-SMA and NID protein levels were assessed by western blot. β-actin was used as a loading control. c RNA was extracted from healthy and SSc fibroblasts. In addition, healthy and SSc fibroblasts were treated with the EZH2 inhibitors GSK126. GLI2 transcript levels were analysed for both inhibitors. Graphs represent the mean and standard error for three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001