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Fig. 5 | Arthritis Research & Therapy

Fig. 5

From: Jo-1 autoantigen-specific B cells are skewed towards distinct functional B cell subsets in anti-synthetase syndrome patients

Fig. 5

JBCs are not increased among transitional B cells or plasmablasts. PBMCs from healthy controls (top) or Jo-1 ARS (bottom) patients were stained and transitional B cells (CD24hi CD38hi CD27 IgD+ CD10+) or plasmablasts (CD24 CD38high) were identified using flow cytometry. a, b Representative plots are shown for healthy control (top) or Jo-1 ARS (bottom) donors for the indicated markers. b CD24hi CD38hi cells as gated in panel a are further gated on IgD/CD27 (left) and the resulting IgD+ CD27 cells are gated on CD10 (right). c, d The indicated phenotypic subsets were examined in n = 5 stable Jo-1 ARS (triangles), n = 5 active Jo-1 ARS (circles), and n = 8 healthy controls (diamonds). Only those Jo-1 ARS patients that had > 20 JBC events were included for JBC sub-analysis of c plasmablasts or d transitional B cells. Individual donors are plotted, and bars represent the mean ± SD. p values were determined using the Mann-Whitney U test, and significant values are indicated on each panel

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