Fig. 5

FICZ reduced CD4⁺IFNγ⁺ and γδ⁺IL-17A⁺ T cells in the lungs at 1 week. BLM at 0.06 units/animal was intratracheally administered with (denoted as FICZ) or without FICZ (vehicle). Single-cell suspensions extracted from lung tissues were stained with anti-mouse CD45, CD3, CD4, γδ, IFN-γ, IL-17A, IL-22, and viability dye 1 week after BLM administration and analyzed by flow cytometry. Summary of the number of CD4⁺ (A) and γδ⁺ T cells (B) producing the cytokines IFN-γ (a), IL-17A (b), and IL-22 (c) was compared between the two groups. n = 5 in each group. *p < 0.05 using Mann-Whitney test