Fig. 4

RvD1 decreased angiogenesis. a Endothelial tube formation assay. Tube formation were observed and photographed in each well (left panel). The number of intersections among branches of assembled HUVEC networks was calculated in the whole field (right panel). b CAM assay. The CAMs were examined and photographed (left panel). The percentage of angiogenic area (right panel) was calculated. n = 10 per group. Control, RA FLS were treated without RvD1; 20 nM RvD1, RA FLS were treated 20 nM RvD1; 100 nM RvD1, RA FLS were treated 100 nM RvD1. 20 ng RvD1, CAM was treated with 20 ng RvD1; 50 ng RvD1, CAM was treated with 50 ng RvD1. All data were represented as the mean ± SD. The differences among three groups were assessed by one-way analysis of variance