Fig. 3
From: MiR-21-5p regulates extracellular matrix degradation and angiogenesis in TMJOA by targeting Spry1
MiR-21-5p directly regulates the expression of Spry1 and affects the expression of specific genes in mandibular condylar chondrocytes (MCCs). a The predicted binding sites of miR-21-5p to the 3′ UTR of Spry1. b Dual-luciferase reporter assay showing that Spry1 is the target gene of miR-21-5p. Asterisk (*): compared with the negative control (NC) group. c Evaluation of the relative microRNA expression of miR-21-5p in MCCs transfected with mimic or inhibitor. RT-qPCR (d) and Western blotting (e) results of cartilage-specific gene expression in MCCs transfected with miR-21-5p mimic. RT-qPCR (f) and Western blotting (g) results of cartilage-specific gene expression in MCCs transfected with miR-21-5p inhibitor. Asterisk (*): compared with the negative control (NC) group. Data are represented as the means ± standard deviation (n = 3). *P < 0.05. NS, not significant