Fig. 8
From: MiR-21-5p regulates extracellular matrix degradation and angiogenesis in TMJOA by targeting Spry1
a Under stimulation with IL-1β, MCCs were transfected with inhibitor NC or miR-21-5p inhibitor for 24 h and then treated with Spry1 siRNA (si-Spry1) for another 24 h. b Under stimulation with IL-1β, MCCs were treated with inhibitor NC or miR-21-5p inhibitor for 24 h and then transfected with the empty vector (cDNA NC) or Spry1 ectopic expression plasmid (cDNA Spry1) for another 24 h. Asterisk (*): compared with the IL-1β + si-NC group (a) or IL-1β + cDNA NC group (b). Hash (#): compared with the IL-1β + si-NC + inhibitor group (a) or IL-1β + cDNA NC + inhibitor group (b). Western blot analysis showing the expression of Spry1, MMP-13, VEGF and p-ERK1/2 in MCCs (a, b). Data are represented as the means ± standard deviation (n = 3). *P < 0.05, #P < 0.05. NS, not significant