Table 1 Sequences of primers used in the real-time polymerase chain reaction
Genes | Sequences of primers | GenBank accession number | Annealing Tm(°C) | Product size (bp) |
|---|---|---|---|---|
SM α2-actin | F: 5′ TATTCTGTCTGGATCGGCGG 3′ R: 5′ ACATTCACAGTTGTGTGCTAGAG 3′ | NM_031004.2 | 60 | 196 |
SM α1-actin | F: 5′ CATGGACCATTATGATTCCCAGC 3′ R: 5′ CTTTGCGCAGGTGGGAGTTG 3′ | NM_031005.3 | 57 | 143 |
SM α22-actin | F: 5′ TCTCCTTCCAGCCCACAAAC3′ R: 5′ TTCACGGCTCATGCCATAGG3′ | NM_031549.2 | 60 | 82 |
h1-calponin | F: 5′ TGGCCCAGAAATACGACCAC3′ R: 5′ CCGGCTGGAGCTTGTTGATA3′ | NM_031747.1 | 60 | 144 |
Nfkb2 | F: 5′ TCTGGTCACCAAGCTCCATGCTAA 3′ R: 5′ TGGATGTCAGCACCAGCCTTTAGA 3′ | NM_001008349.1 | 60 | 129 |
Smooth muscle myosin, heavy chain 11 | F: 5′ TCCGGTGTTCTCCTGCTAGT 3′ R: 5′ GGGCCATTGGGCTGTTTATG 3′ | NM_001170600.1 | 60 | 134 |
β-Actin | F: 5′ TTGCTGACAGGATGCAGAAGGAGA 3′ R: 5′ ACTCCTGCTTGCTGATCCACATCT 3′ | NM_031144.3 | 60 | 159 |