Transcriptome-wide study of TNF-inhibitor therapy in rheumatoid arthritis reveals early signature of successful treatment

Table 3 Transcripts validated in adalimumab good-responders by real-time quantitative polymerase chain reaction (RT-QPCR)

Rank	Target name	Gene	Rq	Rq min	Rq max	Adjusted p value
1	Hs00190574_m1	LIN7A	0.69	0.59	0.8	0.01
2	Hs00191719_m1	CREB5	0.62	0.51	0.76	0.014
3	Hs00969559_m1	ENTPD1	0.79	0.71	0.88	0.019
4	Hs01565750_m1	ITGB7	1.51	1.25	1.82	0.02
5	Hs00185435_m1	HLA-DMA	1.34	1.17	1.52	0.023
6	Hs01075666_m1	IL6R	0.76	0.67	0.87	0.023
7	Hs01062258_m1	SLC8A1	0.68	0.59	0.79	0.025
8	Hs01555410_m1	IL1B	0.65	0.52	0.82	0.027
9	Hs00157950_m1	HLA-DOB	1.98	1.5	2.61	0.03
10	Hs01090216_m1	MGAM	0.56	0.41	0.76	0.03
11	Hs01072219_m1	TRAF5	1.47	1.27	1.69	0.031
12	Hs00171280_m1	AES	1.4	1.24	1.59	0.032
13	Hs00231092_m1	E2F5	1.61	1.26	2.07	0.035
14	Hs00207105_m1	ZFYVE16	0.8	0.71	0.9	0.037
15	Hs01109372_g1	HLA-DOA	1.63	1.3	2.03	0.037
16	Hs00152972_m1	TLR8	0.68	0.55	0.83	0.041
17	Hs00201585_m1	STAP1	1.51	1.22	1.86	0.042

The relative quantification (Rq) values are equivalent to linear fold changes for each transcript defined as the inverse log2 of the change in relative cycle threshold (∆∆Crt) values between pre-treatment and 3 months. Minimum and maximum RQ values and p value are presented for each transcript along with p values adjusted for multiple tests. Transcripts with an Rq value below 1 exhibited reduced expression at 3 months compared to pre-treatment. Transcripts with an Rq value above 1 exhibited increased expression at 3 months compared to pre-treatment

ISSN: 1478-6362