Fig. 2

Lactate import into AF cells. a, ¹⁴C-lactate radioactive tracing to assess lactate import into AF cells. Rabbit AF cell cultures were grown in 1 mM glucose and varying concentrations of ¹⁴C-lactate (1–4 mM) for 1 min, washed with PBS, lysed, and counted in scintillation fluid. b, Lactate enhances MCT1 mRNA expression in AF cells. Rabbit AF cells were cultured under three different conditions, and their MCT1 mRNA levels were measured by qRT-PCR. c, Western blot of MCT-1 and MCT-4 in rabbit AF and NP tissue protein extract (top) and quantification of MCT-1 and MCT-4 expression levels by normalizing to β-actin as loading control (bottom). Data are means ± SEM of four independent experiments (4 rabbits). * p < 0.05