Fig. 1
From: Cold-inducible RNA-binding protein (CIRP) potentiates uric acid-induced IL-1β production
CIRP pretreatment induces MSU-mediated IL-1β synthesis from neutrophils in a dose-dependent manner. Neutrophils (2 × 106/ml) were pretreated with various concentrations of CIRP for 6 h. After pretreatment, the cells were stimulated with or without MSU (200 μg/ml) for 18 h and supernatants were analyzed for IL-1β production by ELISA. Values represent the mean ± SD of two independent experiments. Single asterisk indicates significant difference was observed at a threshold of p < 0.01compared to unstimulated control neutrophils. Double asterisk indicates significant difference was observed at a threshold of p < 0.001compared to unstimulated control neutrophils