Fig. 5
From: Cold-inducible RNA-binding protein (CIRP) potentiates uric acid-induced IL-1β production
Caspase-1 immunoblot analysis using the cellular lysates of MUS-stimulated neutrophils. Neutrophils were pretreated or untreated with the indicated concentrations of CIRP for 6 h. After pretreatment, the cells were stimulated with of MSU (200 μg/ml) for 18 h. Cellular lysates were analyzed by immunoblotting with antibody that recognize cleaved caspase-1 (p20). β-actin was the loading control. Three experiments were performed using different neutrophils, and a representative result is shown