Fig. 3

Knockdown of circ-BRWD1 reversed IL-1β-mediated cell viability, apoptosis, inflammation and ECM degradation in CHON-001 cells. A The expression level of circ-BRWD1 in IL-1β, IL-1β+si-NC, or IL-1β+si-circ-BRWD1 treated CHON-001 cells or untreated cells (control) was determined by qRT-PCR assay. B The viability of CHON-001 cells in different doses of IL-1β-treated CHON-001 cells was evaluated by CCK-8 assay. C–F CHON-001 cells were assigned to 4 groups: control, IL-1β, IL-1β+si-NC, and IL-1β+si-circ-BRWD1. C, D The viability and apoptosis of CHON-001 cells were analyzed by CCK-8 assay and flow cytometry analysis, respectively. E The proliferation of CHON-001 cells was assessed by western blot assay. F The protein levels of CyclinD1 and Bax in CHON-001 cells were measured by western blot assay. G The concentrations of IL-6 and IL-8 in CHON-001 cells were examined by ELISA. H The protein levels of MMP13 and aggrecan in CHON-001 cells were measured by western blot assay. The expression of circ-BRWD1 was examined by 2^-ΔΔCt method with normalization to GAPDH. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001