Fig. 4

Circ-BRWD1 directly targeted miR-1277 and negatively regulated miR-1277 expression in IL-1β-stimulated CHON-001 cells. A The predicted binding sites between circ-BRWD1 and miR-1277. B The luciferase activity in CHON-001 cells co-transfected with miR-NC/miR-1277 and WT-circ-BRWD1/MUT-circ-BRWD1 was measured by dual-luciferase reporter assay. C The enrichment of miR-1277 and circ-BRWD1 in CHON-001 cells was determined by qRT-PCR assay following RIP assay. D, E The expression level of miR-1277 in OA cartilage tissues and IL-1β-stimulated CHON-001 cells was examined by qRT-PCR assay. F The linear correlation between circ-BRWD1 and miR-1277 in OA cartilage tissues was analyzed by Pearson’s correlation coefficient analysis. G The expression level of circ-BRWD1 in IL-1β, IL-1β+pCD5-ciR or IL-1β+circ-BRWD1 treated CHON-001 cells and control cells was detected by qRT-PCR assay. H The level of miR-1277 in IL-1β, IL-1β+si-NC, IL-1β+si-circ-BRWD1, IL-1β+pCD5-ciR, or IL-1β+circ-BRWD1 treated CHON-001 cells and control cells was examined by qRT-PCR assay. The expression of miR-1277 was examined by 2^-ΔΔCt method with normalization to U6. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001