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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Identification of monoclonal antibodies against human renal glomerular endothelial cells in lupus nephritis that induce endothelial interferon-alpha production

Fig. 3

The reactivity of monoclonal antibodies with dsDNA and DNAse 1-treated HRGEC. a Utilizing a commercial IgG anti-dsDNA ELISA kit containing positive and negative controls, the reactivity of LN1–4 and IgG1/IgG3 isotype controls (at the indicated dilution according to the manufacturer’s instructions) with dsDNA was evaluated. The dashed line represents the cutoff, which is equal to the average negative control OD value + 0.1. One of two experiments with similar results is shown. b LN1–3 and IgG1/IgG3 isotype controls were analyzed at the indicated concentrations for the binding to dsDNA. One of two experiments with similar results is shown. c The binding activities of LN1–3 to DNAse I-treated and non-treated HRGEC were assayed by the cell-based ELISA. The mean and SD are given

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Arthritis Research & Therapy

ISSN: 1478-6362

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