Fig. 4
From: Differential impacts of TNFα inhibitors on the transcriptome of Th cells
Comparable binding of TNFis to mTNFα. A–F PBMCs were stimulated with anti-CD3/anti-CD28 and stained with anti-CD69, AF647-ada or AF647-IgG1 at indicated time points. Representative FACS plots of CD4 + T cells are shown in A. Percentages of AF647-ada + Th cells from three experiments are shown in B. Representative FACS plots of CD8+ T cells, B cells, and monocytes are shown in C, D, and E. Unlabeled ada, eta, or cert was used to compete with AF647-ada staining in Th cells (F). Representative histograms from the 24-h time point are shown in the left panel and the MFI of AF647 from two experiments are shown in the right panel. G & H Apoptosis of Th cells from PBMCs pre-stimulated in the presence or absence of indicated TNFis was analyzed with FACS using AnnexinV and 7-AAD. Representative FACS plots (G) and percentages of indicated apoptotic populations (H) from three experiments are shown