Fig. 1

JAK-1/3 and STAT-1/3 expression in synovial tissue of patients with RA. A–D JAK-3 (A) and STAT-1 (C) staining of HC synovial tissue; JAK-3 (B) and STAT-1 (D) staining of RA synovial tissue. E, F The optical density (OD)/area of JAK-3 (E) and STAT-1 (F) were significantly higher in RA synovial tissue when compared with HC synovial tissue. The histogram showed median and range of OD/area for each synovial tissue (**p = 0.006; ***p = 0.0003). G–J JAK-1 (G) and STAT-3 (I) staining of HC synovial tissue; JAK-1 (H) and STAT-3 (J) staining of RA synovial tissue. K, L The fluorescence intensity of JAK-1 (K) and STAT-3 (L) were significantly higher in RA synovial tissue, when compared with HC synovial tissue. The histograms showed median and range of fluorescence intensity of each synovial tissues (**p ≤ 0.0002). M, N VEGF (green) and vWF (red) staining of HC (M) synovial tissue and RA (N) synovial tissue. O, P The fluorescence intensity of VEGF (O) and the number of vessel expressing vWF (P) were significantly higher in RA synovial tissue, when compared with HC synovial tissue. The histograms showed median and range of each synovial tissue (*p = 0.02; **p = 0.001). Original magnification ×20