Fig. 5

Tregs from young and old mice have similar suppressive capacities. a, b In 3 independent experiments, CD25⁺CD4⁺ regulatory T cells were sorted from young (average age <12 weeks, n=8) or old (average age >81 weeks, n=9) mice. Tregs were cocultured with CFSE-labeled CD25^-CD62L⁺CD44^-CD4⁺ cells from young mice in the presence of splenic conventional DCs from young mice and soluble anti-CD3ε antibodies for 3 days. CFSE dilution was analyzed (a), and the proliferation index was calculated as a measure representing the proliferation cycles/starting cell. Different ratios of Tregs and Th cells were analyzed as shown in the diagrams (b). The “noTreg” control is identical in all ratio combination of Tregs-Th cell ratios displayed. Statistical testing was performed as described in the methods section. n.s. non-significant