The citrullinated/native index of autoantibodies against hnRNP-DL predicts an individual “window of treatment success” in RA patients

Table 2 Frequency of autoantibodies against recombinant hnRNP-DL_mir in sera from different RA and SLE mouse models

Mouse model	Model	Autoantigen(s) assayed	No. of sera tested	% Positive	Ratio, Mean OD positive^b
SKG (-/+ Zymosan)	RA	hnRNP-DL_mir	8/8	25/50	2.48/1.23
Balb/c (IL-1Ra^−/−)	RA	hnRNP-DL_mir	36	100	7.89
MRL-lpr	SLE	hnRNP-DL_mir^a	20	85	4.22
MRL-lpr (MyD88^−/−)	SLE	hnRNP-DL_mir^a	20	10	1.3
MRL-lpr (TLR9^−/−)	SLE	hnRNP-DL_mir	4	50	2.42
MRL-lpr (TLR7^−/−)	SLE	hnRNP-DL_mir	7	43	2.55
MRL-lpr (TLR7/9^−/−)	SLE	hnRNP-DL_mir	7	0	-
C57BL/6 lpr	SLE	hnRNP-DL_mir^a	12	33	2.46
C57BL/6 lpr (SIGIRR/TIR8^−/−)	SLE	hnRNP-DL_mir^a	12	83	6.83
C57BL/6 (-/+ R848)	TLR7/8 agonist	hnRNP-DL_mir	10/10	0/10	-/1.39

RA rheumatoid arthritis, SLE systemic lupus erythematosus, SIGIRR/TIR8 Single Ig IL-1-related receptor/Toll/interleukin-1 receptor 8, R848 SIGIRR TLR7/8 agonist, MyD88 myeloid differentiation primary response gene 88, TLR Toll-like receptor
^aAdditionally citrullinated hnRNP-DL_mir were tested. In no case, citrullination of hnRNP-DL_mir resulted in a higher signal compared to the native hnRNP-DL_mir form (no additional reactivity)
^bRatio mean OD positive reflects the level of the positive signals in each mouse model and was calculated as the quotient of the mean value of the positive signals and the diagnostic cutoff

ISSN: 1478-6362