Fig. 1

AxSpA signature in monocyte transcriptomes as compared to HC. Monocytes from HLA-B27+ patients with axSpA (n = 25) and healthy controls (HC, n = 10) of cohort 1 were subjected to GeneChip microarray analysis, which resulted in 957 significant probe-sets according to HPCDA scoring (HPCDA score > 100). A 957 significant probe-sets (296 down- and 661 upregulated) shown with HPCDA-volcano plot (red circles). B Principal component analysis (PCA) of 25 axSpA patients and 10 HC using the 957 significant probe-sets shows 38%, 8%, and 7% variance in the first 3 principal components 1 (PC1), 2 (PC2), and 3 (PC3). C Hierarchical clustering of 25 axSpA patients and 10 HC according to 957 significant probe-sets. Rows represent probe-set, and columns represent individual samples. Row values are color-coded according to their normalized signal intensities (max + 2, min − 2) as indicated by the scale bar (green lower or red color higher than probe-set mean). D Gene set enrichment analysis (GSEA) using MSigDB as input data base was performed for the gene lists of upregulated genes (upper panel) and downregulated genes (lower panel) of the axSpA signature of cohort 1 and 2