Fig. 7

ACSM5 regulates the proliferation, fibrosis, and apoptosis of LFCs. A qPCR and Western Blot results showing the expression level of ACSM5 in LFCs from HLF patients with the treatment of pCMV-ACSM5 or empty plasmid. B CCK8 assays of LFCs from HLF patients with the treatment of pCMV-ACSM5 or empty plasmid. Absorbances were measured at 24 h, 48 h, 72 h, and 96 h post cell seeding. C Flow graph and statistical results of apoptosis assays using LFCs from HLF patients with the treatment of pCMV-ACSM5 or empty plasmid. D The relative caspase3 activity of LFCs from HLF patients with the treatment of pCMV-ACSM5 or empty plasmid. E qPCR results showing the expression level of COL1, COL3, MMP13, and MMP2 in LFCs from HLF patients with the treatment of pCMV-ACSM5 or empty plasmid. F Western Blot results showing the expression level of COL1, COL3, MMP13, and MMP2 in LFCs from HLF patients with the treatment of pCMV-ACSM5 or empty plasmid. Data were presented as the mean ± SME of three independent experiments. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001